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Anti-Ustekinumab Polyclonal Antibody (AB769014)

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Overview
Catalog No.AB769014
Description
Anti-Ustekinumab Polyclonal Antibody (AB769014) is a rabbit polyclonal antibody detecting Ustekinumab in ELISA.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
ApplicationsELISA
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen Ustekinumab
Target Rabbit polyclonal to Stelara.
Specificity The product is specific for Ustekinumab. This antibody serves as an excellent positive control for Ustekinumab immunogenicity (ADA) assays.
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession CAS: 815610-63-0
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage The product can be stored for 2 - 3 weeks at 2 to 8°C or for up to 12 months at -20°C. Avoid repeated freezing and thawing cycles.
BackgroundUstekinumab with trade name Stelara, is an FDA-approved drug to Crohn's disease, Ulcerative Colitis, Plaque Psoriasis and Psoriatic Arthritis, targeting both interleukins (IL)-12 and IL-23. Ustekinumab is a fully human immunoglobulin G1 kappa mAb that binds to IL-12 and IL-23. Ustekinumab binds to the p40 subunit common to IL-12 and IL-23 and prevents their interaction with the IL-12 receptor β1 subunit of the IL-12 and IL-23 receptor complexes, thereby disrupting the proinflammatory pathway. Antibodysystem Anti-Ustekinumab Antibody, pAb, Rabbit is produced from sera of a rabbit immunized with Ustekinumab.
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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