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Anti-UXT Polyclonal Antibody (HV545014)

Anti-UXT Polyclonal Antibody
Anti-UXT Polyclonal Antibody
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Overview
Catalog No.HV545014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human UXT (Glu15-His157).
Target ART-27, Protein UXT, Ubiquitously expressed transcript protein, Androgen receptor trapped clone 27 protein, UXT
Purification Purified by antigen affinity column.
Accession Q9UBK9
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Protein UXT is a ~18 kDa protein. Involved in gene transcription regulation. Acts in concert with the corepressor URI1 to regulate androgen receptor AR-mediated transcription. Together with URI1, associates with chromatin to the NKX3-1 promoter region. Negatively regulates the transcriptional activity of the estrogen receptor ESR1 by inducing its translocation into the cytoplasm. May act as nuclear chaperone that facilitates the formation of the NF-kappa-B enhanceosome and thus positively regulates NF-kappa-B transcription activity.

1. Mita, P. et al. (2011) Molecular and cellular biology 31, 3639-52. PMID: 21730289
2. Sánchez-Morgan, N. et al. (2017) Journal of cellular biochemistry 118, 2347-2356. PMID: 28106301
3. Markus, SM. et al. (2002) Molecular biology of the cell 13, 670-82. PMID: 11854421
4. Sun, S. et al. (2007) The Journal of cell biology 178, 231-44. PMID: 17620405
5. Huang, Y. et al. (2011) Molecular biology of the cell 22, 1389-97. PMID: 21307340
6. Moss, TN. et al. (2007) In vitro cellular & developmental biology. Animal 43, 139-46. PMID: 17554592
Note For research use only.
Images
  • Anti-UXT Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-UXT polyclonal antibody at 1ug/mL on recombinant UXT (Catalog No. HV545012).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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