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Anti-Vaccinia A27 Recombinant Antibody (8E3#) (VK519063)

Anti-Vaccinia A27 Recombinant Antibody (8E3#)
Anti-Vaccinia A27 Recombinant Antibody (8E3#)
Anti-Vaccinia A27 Recombinant Antibody (8E3#)
Anti-Vaccinia A27 Recombinant Antibody (8E3#)
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Overview
Catalog No.VK519063
Species reactivityVaccinia
ApplicationsELISA, FCM, WB
Host speciesHuman
IsotypeIgG1, kappa
Clone ID8E3#
Clonality Monoclonal
Target Protein A27, VACWR150, A27L
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P11258
RRID Anti-Vaccinia A27 Antibody (8E3#) (abinScience Cat# VK519063, RRID:AB_3727112)
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Protein OPG154 (A27L) is a ~12 kDa protein. Structural protein involved in the envelopment of mature virion (MV) to form the wrapped virion (WV). The wrapping consists of the addition of Golgi membranes to the mature virion. Participates in mature virion (MV) movement within the infected cell. May play an indirect role in MV-cell fusion.

Note For research use only.
Images
  • Anti-Vaccinia A27 Recombinant Antibody (8E3#)

    SDS-PAGE

    SDS-PAGE for Anti-Vaccinia A27 Antibody (8E3#).

  • Anti-Vaccinia A27 Recombinant Antibody (8E3#)

    Western blot

    Western blot analysis was performed using anti-A29L monoclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant monkeypox virus A29L (Catalog No: VK415011)
    Lane 2: negative control

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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