Anti-Vaccinia A33R Recombinant Antibody (A27D7) (VK465023)

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Overview

Catalog No.

VK465023

Description

Anti-Vaccinia A33R Antibody (A27D7) [A27D7] (VK465023) is a human monoclonal antibody detecting A33R in ELISA, Neutralization, WB, IHC, IF. Suitable for Vaccinia.

Highlights

●Multi-Application — Validated across multiple applications.

Species reactivity

Vaccinia

Applications

ELISA, Neutralization, WB, IHC, IF

Host species

Human

Isotype

IgG1, kappa

Clone ID

A27D7

Clonality

Monoclonal

Target

Protein A33, VACWR156, A33R

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P68617

RRID

Anti-Vaccinia A33R Antibody (A27D7) (abinScience Cat# VK465023, RRID:AB_3727113)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Protein OPG161 (A33R) is a ~20 kDa protein. Forms a complex with OPG162 and OPG190 to coordinate the incorporation of OPG164 into wrapped enveloped virion (EV) membranes and, subsequently, the production of actin tails. Therefore plays an essential role in efficient cell-to-cell spread of viral particles.

1. Wolffe, EJ. et al. (2001) Journal of virology 75, 303-10. PMID: 11119600

2. Breiman, A. et al. (2013) The Journal of general virology 94, 720-725. PMID: 23255618

3. Monticelli, SR. et al. (2020) Journal of virology 94. PMID: 31941777

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Vaccinia A33R Antibody (A27D7).

References

Datasheet

Anti-Vaccinia A33R Recombinant Antibody (A27D7).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Vaccinia A33R Recombinant Antibody (A27D7) (VK465023)

Overview

Images

References

Datasheet

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