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Anti-VAMP8 Polyclonal Antibody (HP094024)

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Overview
Catalog No.HP094024
Description
Anti-VAMP8 Polyclonal Antibody (HP094024) is a rabbit polyclonal antibody detecting Vesicle-associated membrane protein 8; VAMP-8; Endobrevin; EDB; VAMP8 in ELISA, IHC, WB. Suitable for Human, Rat, Bovine, and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human VAMP8 (Glu2-Lys75).
Target Vesicle-associated membrane protein 8; VAMP-8; Endobrevin; EDB; VAMP8
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q9BV40, Q9WUF4, Q3T0Y8, O70404
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Vesicle-associated membrane protein 8 (VAMP8) is a ~11 kDa protein. SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex. Also required for dense-granule secretion in platelets. Also plays a role in regulated enzyme secretion in pancreatic acinar cells.

1. Itakura, E. et al. (2012) Cell 151, 1256-69. PMID: 23217709
2. Diao, J. et al. (2015) Nature 520, 563-6. PMID: 25686604
3. Polgár, J. et al. (2002) Blood 100, 1081-3. PMID: 12130530
4. van Tol, S. et al. (2020) Journal of virology 94. PMID: 31694946
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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V₁ (Stock Vol.)
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C₂ (Working Conc.)
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Anti-VAMP8 Polyclonal Antibody

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