Anti-VDAC1 Polyclonal Antibody (HB031014)

Name: Anti-VDAC1 Polyclonal Antibody
Brand: abinScience
SKU: HB031014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HB031014

Description

Anti-VDAC1 Polyclonal Antibody (HB031014) is a rabbit polyclonal antibody detecting VDAC1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human VDAC1 (Met1-Ala283).

Target

Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, hVDAC1, Porin 31HL, Porin 31HM, Voltage-dependent anion-selective channel protein 1, VDAC1, VDAC-1, VDAC

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P21796, Q60932, Q9Z2L0

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Non-selective voltage-gated ion channel VDAC1 is a ~30 kDa protein. Non-selective voltage-gated ion channel that mediates the transport of anions and cations through the mitochondrion outer membrane and plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. Binds various signaling molecules, including the sphingolipid ceramide, the phospholipid phosphatidylcholine, and the sterols cholesterol and oxysterol.

1. Stadtmüller, U. et al. (1999) Biological chemistry 380, 1461-6. PMID: 10661876

2. Thinnes, FP. et al. (2001) Pflugers Archiv : European journal of physiology 443 Suppl 1, S111-6. PMID: 11845315

3. Hiller, S. et al. (2008) Science (New York, N.Y.) 321, 1206-10. PMID: 18755977

4. Marginedas-Freixa, I. et al. (2018) Scientific reports 8, 11384. PMID: 30061676

5. Blachly-Dyson, E. et al. (1993) The Journal of biological chemistry 268, 1835-41. PMID: 8420959

6. Dadsena, S. et al. (2019) Nature communications 10, 1832. PMID: 31015432

7. Ham, SJ. et al. (2020) Proceedings of the National Academy of Sciences of the United States of America 117, 4281-4291. PMID: 32047033

10. Chen, Y. et al. (2010) Biochemical and biophysical research communications 394, 798-803. PMID: 20230784

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-VDAC1 polyclonal antibody at 1µg/ml on various samples.
Lane 1: HepG2 cell lysate
Lane 2: Hela cell lysate
Lane 3: Mouse brain lysate
Lane 4: Rat brain lysate
Lane 5: Mouse heart lysate
Lane 6: Rat heart lysate

References

Datasheet

Anti-VDAC1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

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Anti-VDAC1 Polyclonal Antibody (HB031014)

Overview

Images

References

Datasheet

SDS

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