Anti-VEEV Spike glycoprotein Recombinant Nanobody (SAA2750) (VK039043)

Name: Anti-VEEV Spike glycoprotein Recombinant Nanobody (SAA2750)
Brand: abinScience
SKU: VK039043
Price: 191 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

VK039043

Description

Anti-VEEV Spike glycoprotein Nanobody (SAA2750) [SAA2750] (VK039043) is a alpaca monoclonal antibody detecting Spike glycoprotein E1 in ELISA.

Species reactivity

Venezuelan equine encephalitis virus (VEEV)

Applications

ELISA

Host species

Alpaca

Isotype

VHH-His

Clone ID

SAA2750

Clonality

Monoclonal

Target

6K protein, Assembly protein E3, C, Capsid protein, Coat protein, E1 envelope glycoprotein, E2 envelope glycoprotein, EC:3.4.21.90, Precursor of protein E3/E2, Spike glycoprotein E1, Spike glycoprotein E2, Structural polyprotein, p130, p62, pE2

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Accession

P05674

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Structural polyprotein (Spike glycoprotein) is a ~138 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions.

Note

For research use only

Images

References

Datasheet

Anti-VEEV Spike glycoprotein Recombinant Nanobody (SAA2750).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-VEEV Spike glycoprotein Recombinant Nanobody (SAA2750) (VK039043)

Overview

Images

References

Datasheet

SDS

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