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Anti-Vibrio cholerae serotype O1 ctxB/Cholera Toxin Subunit B Polyclonal Antibody (JN074014)

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Overview
Catalog No.JN074014
Description
Anti-Vibrio cholerae serotype O1 ctxB/Cholera Toxin Subunit B Polyclonal Antibody (JN074014) is a rabbit polyclonal antibody detecting Cholera enterotoxin subunit B in ELISA, IHC, WB.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Vibrio cholerae serotype O1 ctxB/Cholera Toxin Subunit B (Thr22-Asn124).
Target Cholera enterotoxin subunit B, Cholera enterotoxin B chain, Cholera enterotoxin gamma chain, Choleragenoid, ctxB, toxB, VC_1456
Purification Purified by antigen affinity column.
Accession P01556
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Cholera enterotoxin subunit B (ctxB) is a ~13 kDa protein. The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself.

Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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