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Anti-VIP Recombinant Antibody (L108/92) (HF589023)

Anti-VIP Recombinant Antibody (L108/92)
Anti-VIP Recombinant Antibody (L108/92)
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Overview
Catalog No.HF589023
Description
Anti-VIP Recombinant Antibody (L108/92) (HF589023) is a mouse monoclonal antibody detecting VIP in IHC, WB. Suitable for Human, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Rat
ApplicationsIHC, WB
Host speciesMouse
ClonalityMonoclonal
IsotypeIgG2a, kappa
Clone IDL108/92
Target Vasoactive intestinal polypeptide, Peptide histidine valine 42, VIP, Peptide histidine methioninamide 27, VIP peptides
Endotoxin level < 10 EU/mg as determined by LAL method.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P01282 ,P01283
RRID

Anti-VIP Antibody (L108/92) (abinScience Cat# HF589023, RRID:AB_3728090)
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Form Liquid
Storage buffer 0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Product Usage Information
Application Dilution
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

VIP peptides is a ~19 kDa protein. VIP is a neuropeptide involved in a diverse array of physiological processes through activating the PACAP subfamily of class B1 G protein-coupled receptors: VIP receptor 1 (VPR1) and VIP receptor 2 (VPR2). Abundantly expressed throughout the CNS and peripheral nervous systems where they primarily exert neuroprotective and immune modulatory roles. Also causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder.

1. Kitamura, K. et al. (1992) Biochemical and biophysical research communications 185, 134-41. PMID: 1318039
2. Piper, SJ. et al. (2022) Nature communications 13, 7013. PMID: 36385145
3. Wei, Y. et al. (1996) Journal of neuroendocrinology 8, 811-7. PMID: 8933357
4. Brenneman, DE. et al. (1986) Proceedings of the National Academy of Sciences of the United States of America 83, 1159-62. PMID: 3456568
5. Ma, JN. et al. (2004) Biochemical pharmacology 67, 1279-84. PMID: 15013843
Note For research use only.
Images
  • Anti-VIP Recombinant Antibody (L108/92)

    SDS-PAGE

    SDS-PAGE for Anti-VIP Antibody (L108/92)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Molecular Weight *
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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