Anti-VSV Nucleoprotein Polyclonal Antibody (VK422064)

Name: Anti-VSV Nucleoprotein Polyclonal Antibody
Brand: abinScience
SKU: VK422064
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

VK422064

Description

Anti-VSIV Nucleoprotein/NP Polyclonal Antibody (VK422064) is a rabbit polyclonal antibody detecting Nucleoprotein in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Vesicular stomatitis Indiana virus (VSIV)

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant VSIV Nucleoprotein/NP (Ser2-Lys422).

Target

N, NP, Nucleocapsid protein, Nucleoprotein, Protein N

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P03521

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Nucleoprotein (NP) is a ~47 kDa protein. Encapsidates the genome in a ratio of one N per nine ribonucleotides, protecting it from nucleases. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. The nucleocapsid is bullet-shaped with the tip containing 8 turns of a conical spiral before reaching the helical cylindrical trunk. Nucleocapsid assembly is concomitant with replication, therefore viral replication depends on the intracellular concentration of free N, termed N(0). All replicative products are resistant to nucleases.

1. Green, TJ. et al. (2006) Science (New York, N.Y.) 313, 357-60. PMID: 16778022

2. Ge, P. et al. (2010) Science (New York, N.Y.) 327, 689-93. PMID: 20133572

3. Gumpper, RH. et al. (2018) Journal of virology 92. PMID: 29437970

4. Jenni, S. et al. (2022) Nature communications 13, 4802. PMID: 35970826

5. Zhou, K. et al. (2022) Nature communications 13, 5980. PMID: 36216930

6. Green, TJ. et al. (2011) Journal of virology 85, 2714-22. PMID: 21177817

7. Si, Z. et al. (2022) Proceedings of the National Academy of Sciences of the United States of America 119, e2111948119. PMID: 35476516

Note

For research use only

Images

References

Datasheet

Anti-VSV Nucleoprotein Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-VSV Nucleoprotein Polyclonal Antibody (VK422064)

Overview

Images

References

Datasheet

SDS