Anti-VSIV RdRp/Protein L Polyclonal Antibody (VK422034)

Datasheet Share product Copy info

Price(USD): $

Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

VK422034

Description

Anti-VSIV RdRp/Protein L Polyclonal Antibody (VK422034) is a rabbit polyclonal antibody detecting VSIV RdRp/Protein L in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Vesicular stomatitis Indiana virus

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant VSIV RdRp/Protein L (Glu2-Gly188).

Target

polymerase, partial, polymerase, partial

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q8B0I0, Q8B0H5, Q8B0H0, Q98776, P03523

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

RNA-directed RNA polymerase L (RdRp/Protein L) is a ~240 kDa protein. Multifunctional enzyme responsible for RNA synthesis (replicase and transcriptase), cap addition, and cap methylation. Also performs the polyadenylation of subgenomic mRNAs by a stuttering mechanism at a slipery stop site present at the end of viral genes. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). L is packaged into virions during assembly and translocates to the 3' leader promoter to initiate transcription after entering the host cells. During transcription and replication of the genome, L does not bind the N-RNA complex directly, but is bridged by its non-catalytic cofactor P, which interacts with L and N oligomers simultaneously.

Note

For research use only

Images

References

Datasheet

Anti-VSIV RdRp/Protein L Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-VSIV RdRp/Protein L Polyclonal Antibody (VK422034)

Overview

Images

References

Datasheet

Quick access

Contact Us