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Anti-WDFY1 Polyclonal Antibody (HV550014)

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Overview
Catalog No.HV550014
Description
Anti-WDFY1 Polyclonal Antibody (HV550014) is a rabbit polyclonal antibody detecting WDFY1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human WDFY1 (Met1-His410).
Target FENS-1, Zinc finger FYVE domain-containing protein 17, WD40- and FYVE domain-containing protein 1, FYVE domain-containing protein localized to endosomes 1, KIAA1435, ZFYVE17, WDFY1, FENS1, WD repeat and FYVE domain-containing protein 1, Phosphoinositide-binding protein 1, WDF1
Purification Purified by antigen affinity column.
Accession Q8IWB7
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

WD repeat and FYVE domain-containing protein 1 (WDFY1) is a ~46 kDa protein. Positively regulates TLR3- and TLR4-mediated signaling pathways by bridging the interaction between TLR3 or TLR4 and TICAM1. Promotes TLR3/4 ligand-induced activation of transcription factors IRF3 and NF-kappa-B, as well as the production of IFN-beta and inflammatory cytokines.

1. Hu, YH. et al. (2015) EMBO reports 16, 447-55. PMID: 25736436
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-WDFY1 Polyclonal Antibody

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