Anti-WDR82 Polyclonal Antibody (HB354014)

Name: Anti-WDR82 Polyclonal Antibody
Brand: abinScience
SKU: HB354014
Price: 168 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HB354014

Description

Anti-WDR82 Polyclonal Antibody (HB354014) is a rabbit polyclonal antibody detecting WD repeat-containing protein 82; WDR82; SWD2; TMEM113; WDR82A; UNQ9342/PRO34047 in ELISA, IHC, WB. Suitable for Human, Mouse, Chicken, Frog, and Zebrafish.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant human WDR82 (Met1-Asp313).

Target

WD repeat-containing protein 82; WDR82; SWD2; TMEM113; WDR82A; UNQ9342/PRO34047

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q6UXN9, Q8BFQ4, Q5ZMV7, Q58E77, Q640J6, Q6NV31

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

WD repeat-containing protein 82 (WDR82) is a ~35 kDa protein. Regulatory component of the SET1/COMPASS complex implicated in the tethering of this complex to transcriptional start sites of active genes. Facilitates histone H3 'Lys-4' methylation (H3K4me) via recruitment of the SETD1A or SETD1B to the 'Ser-5' phosphorylated C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A). Component of the PNUTS-PP1 protein phosphatase complex, a protein phosphatase 1 (PP1) complex that promotes RNA polymerase II transcription pause-release, allowing transcription elongation. PNUTS-PP1 also plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Together with ZC3H4, but independently of the SET1 complex, part of a transcription termination checkpoint that promotes transcription termination of long non-coding RNAs (lncRNAs).

1. Lee, JH. et al. (2008) Molecular and cellular biology 28, 609-18. PMID: 17998332

2. Wu, M. et al. (2008) Molecular and cellular biology 28, 7337-44. PMID: 18838538

3. Lee, JH. et al. (2010) The Journal of biological chemistry 285, 24466-76. PMID: 20516061

4. Wang, Z. et al. (2024) Molecular cell 84, 4824-4842.e7. PMID: 39603240

5. Kelley, JR. et al. (2024) Molecular cell 84, 4843-4861.e8. PMID: 39603239

6. Austenaa, LMI. et al. (2021) Nature structural & molecular biology 28, 337-346. PMID: 33767452

7. Estell, C. et al. (2021) eLife 10. PMID: 33913806

Note

For research use only

Images

References

Datasheet

Anti-WDR82 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-WDR82 Polyclonal Antibody (HB354014)

Overview

Images

References

Datasheet

SDS