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Anti-WNT5A Polyclonal Antibody (MW294014)

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Overview
Catalog No.MW294014
Description
Anti-WNT5A Polyclonal Antibody (MW294014) is a rabbit polyclonal antibody detecting WNT5A in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Alopias vulpinus, and Ambystoma mexicanum.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse WNT5A (Ile62-Lys380).
Target Protein Wnt-5a, WNT5A
Purification Purified by antigen affinity column.
Accession P22725
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Protein Wnt-5a (WNT5A) is a ~42 kDa protein. Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation.

1. Kurayoshi, M. et al. (2007) The Biochemical journal 402, 515-23. PMID: 17117926
2. Mikels, AJ. et al. (2006) PLoS biology 4, e115. PMID: 16602827
3. Yamaguchi, TP. et al. (1999) Development (Cambridge, England) 126, 1211-23. PMID: 10021340
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-WNT5A Polyclonal Antibody

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