Anti-XPA Polyclonal Antibody (HA613014)

Name: Anti-XPA Polyclonal Antibody
Brand: abinScience
SKU: HA613014
Price: 96 USD
Availability: InStock

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Overview

Catalog No.

HA613014

Description

Anti-XPA Polyclonal Antibody (HA613014) is a rabbit polyclonal antibody detecting DNA repair protein complementing XP-A cells; Xeroderma pigmentosum group A-complementing protein; XPA; XPAC in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant human XPA (Asp101-Met273).

Target

DNA repair protein complementing XP-A cells; Xeroderma pigmentosum group A-complementing protein; XPA; XPAC

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P23025, Q64267

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

DNA repair protein complementing XP-A cells (XPA) is a ~31 kDa protein. Involved in DNA nucleotide excision repair (NER). Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation. During NER stimulates the 5'-3' helicase activity of XPD/ERCC2 and the DNA translocase activity of XPB/ERCC3. Connects XPD/ERCC2 and XPB/ERCC3 during NER, retaining DNA near the XPB/ERCC3 active site, and stabilizing the complex in a different conformation than in transcribing TFIIH.

1. Pan, YR. et al. (2009) Cell cycle (Georgetown, Tex.) 8, 655-64. PMID: 19197159

2. Kokic, G. et al. (2019) Nature communications 10, 2885. PMID: 31253769

Note

For research use only

Images

References

Datasheet

Anti-XPA Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-XPA Polyclonal Antibody (HA613014)

Overview

Images

References

Datasheet

SDS

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