Anti-XPNPEP3 Polyclonal Antibody (HV814014)

Name: Anti-XPNPEP3 Polyclonal Antibody
Brand: abinScience
SKU: HV814014
Price: 96 USD
Availability: InStock

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References
Datasheet
SDS

Overview

Catalog No.

HV814014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human XPNPEP3 (Glu253-Gln482).

Target

Xaa-Pro aminopeptidase 3, APP3, XPNPEP3, Aminopeptidase P3, X-Pro aminopeptidase 3

Purification

Purified by antigen affinity column.

Accession

Q9NQH7

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Xaa-Pro aminopeptidase 3 (XPNPEP3) is a ~57 kDa protein. Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Leu-Pro-Ala. Also shows low activity towards peptides with Ala or Ser at the P1 position.

1. Inoue, M. et al. (2015) Journal of cell science 128, 656-69. PMID: 25609706

2. Singh, R. et al. (2017) The Journal of biological chemistry 292, 10035-10047. PMID: 28476889

Note

For research use only.

Images

Immunofluorescence

A549 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting XPNPEP3 (HV814014) at a concentration of 41.5 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of XPNPEP3 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Western blot

Western blot analysis was performed using anti-XPNPEP3 polyclonal antibody at 1μg/ml on various samples.
Lane 1: 293T cell lysate
Lane 2: HepG2 cell lysate
Lane 3: PC3 cell lysate
Lane 4: Mouse kidney lysate
Lane 5: Mouse heart lysate

References

Datasheet

Anti-XPNPEP3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-XPNPEP3 Polyclonal Antibody (HV814014)

Overview

Images

References

Datasheet

SDS

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