Anti-XRCC1 Polyclonal Antibody (HB640014)

Name: Anti-XRCC1 Polyclonal Antibody
Brand: abinScience
SKU: HB640014
Price: 96 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

20ug
50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HB640014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human XRCC1 (Thr284-Ala633).

Target

DNA repair protein XRCC1, XRCC1, X-ray repair cross-complementing protein 1

Purification

Purified by antigen affinity column.

Accession

P18887

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

DNA repair protein XRCC1 is a ~69 kDa protein. Scaffold protein involved in DNA single-strand break repair by mediating the assembly of DNA break repair protein complexes. Negatively regulates ADP-ribosyltransferase activity of PARP1 during base-excision repair in order to prevent excessive PARP1 activity. Recognizes and binds poly-ADP-ribose chains: specifically binds auto-poly-ADP-ribosylated PARP1, limiting its activity.

1. Whitehouse, CJ. et al. (2001) Cell 104, 107-17. PMID: 11163244

2. Hoch, NC. et al. (2017) Nature 541, 87-91. PMID: 28002403

3. Demin, AA. et al. (2021) Molecular cell 81, 3018-3030.e5. PMID: 34102106

4. Adamowicz, M. et al. (2021) Nature cell biology 23, 1287-1298. PMID: 34811483

5. El-Khamisy, SF. et al. (2003) Nucleic acids research 31, 5526-33. PMID: 14500814

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-XRCC1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: K562 cell lysate
Lane 3: Mouse testis lysate

References

Datasheet

Anti-XRCC1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-XRCC1 Polyclonal Antibody (HB640014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us