Anti-Zaire ebolavirus NP/Nucleoprotein Polyclonal Antibody (VK512014)

Name: Anti-Zaire ebolavirus NP/Nucleoprotein Polyclonal Antibody
Brand: abinScience
SKU: VK512014
Price: 168 USD
Availability: InStock

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Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

VK512014

Species reactivity

Zaire ebolavirus

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Zaire ebolavirus NP/Nucleo(Val36-Glu351).

Target

Nucleoprotein, Nucleocapsid protein, NP

Purification

Purified by antigen affinity column.

Accession

O72142, P18272

RRID

Anti-Zaire ebolavirus NP/Nucleoprotein Polyclonal Antibody (abinScience Cat# VK512014, RRID:AB_3730853)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Nucleoprotein (NP) is a ~83 kDa protein. Oligomerizes into helical capsid to encapsidate the viral genome, protecting it from nucleases and the cellular innate immune response. VP35 binds to and stabilizes monomeric NP, keeping it soluble. Upon virus replication, NP is recruited to bind cooperatively viral genomic RNA and VP35 is released. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. The nucleocapsid is helical with a pitch of 10.81 NP per turn and a diameter of about 22nm.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Ebola virus Nucleoprotein polyclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant Zaire ebolavirus NP/Nucleoprotein (Catalog No: VK512012), 70 ug
Lane 2: recombinant Zaire ebolavirus NP/Nucleoprotein (Catalog No: VK512012), 50 ug
Lane 3: recombinant Zaire ebolavirus NP/Nucleoprotein (Catalog No: VK512012), 30 ug
Lane 4: recombinant Zaire ebolavirus NP/Nucleoprotein (Catalog No: VK512012), 10 ug

References

Datasheet

Anti-Zaire ebolavirus NP/Nucleoprotein Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Zaire ebolavirus NP/Nucleoprotein Polyclonal Antibody (VK512014)

Overview

Images

References

Datasheet

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