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Anti-ZBTB16 Polyclonal Antibody (HC522014)

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Overview
Catalog No.HC522014
Description
Anti-ZBTB16 Polyclonal Antibody (HC522014) is a rabbit polyclonal antibody detecting Zinc finger and BTB domain-containing protein 16; Promyelocytic leukemia zinc finger protein; Zinc finger protein 145; Zinc finger protein PLZF; ZBTB16; PLZF; ZNF145 in ELISA, IHC, WB. Suitable for Human and Zebrafish.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human ZBTB16 (Ile402-Val673).
Target Zinc finger and BTB domain-containing protein 16; Promyelocytic leukemia zinc finger protein; Zinc finger protein 145; Zinc finger protein PLZF; ZBTB16; PLZF; ZNF145
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q05516, Q802Y8
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Zinc finger and BTB domain-containing protein 16 (ZBTB16) is a ~74 kDa protein. Acts as a transcriptional repressor. Transcriptional repression may be mediated through recruitment of histone deacetylases to target promoters. May play a role in myeloid maturation and in the development and/or maintenance of other differentiated tissues. Probable substrate-recognition component of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.

1. Melnick, AM. et al. (2000) Molecular and cellular biology 20, 2075-86. PMID: 10688654
2. Lin, DY. et al. (2013) Journal of biomedical science 20, 98. PMID: 24359566
3. Furukawa, M. et al. (2003) Nature cell biology 5, 1001-7. PMID: 14528312
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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