Anti-ZDHHC5 Polyclonal Antibody (HA293014)

Name: Anti-ZDHHC5 Polyclonal Antibody
Brand: abinScience
SKU: HA293014
Price: 168 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HA293014

Description

Anti-ZDHHC5 Polyclonal Antibody (HA293014) is a rabbit polyclonal antibody detecting ZDHHC5 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human ZDHHC5 (Ser63-Tyr148).

Target

ZDHHC5, Palmitoyltransferase ZDHHC5, DHHC-5, Zinc finger DHHC domain-containing protein 5, ZNF375, KIAA1748, Zinc finger protein 375

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q9C0B5

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Palmitoyltransferase ZDHHC5 is a ~77 kDa protein. Palmitoyltransferase that catalyzes the addition of palmitate onto various protein substrates such as CTNND2, CD36, GSDMD, NLRP3, NOD1, NOD2, STAT3 and S1PR1 thus plays a role in various biological processes including cell adhesion, inflammation, fatty acid uptake, bacterial sensing or cardiac functions. Plays an important role in the regulation of synapse efficacy by mediating palmitoylation of delta-catenin/CTNND2, thereby increasing synaptic delivery and surface stabilization of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors (AMPARs). Under basal conditions, remains at the synaptic membrane through FYN-mediated phosphorylation that prevents association with endocytic proteins. Neuronal activity enhances the internalization and trafficking of DHHC5 from spines to dendritic shafts where it palmitoylates delta-catenin/CTNND2. Regulates cell adhesion at the plasma membrane by palmitoylating GOLGA7B and DSG2.

1. Kokkola, T. et al. (2011) FEBS letters 585, 2665-70. PMID: 21820437

2. Badawy, SMM. et al. (2017) Scientific reports 7, 16552. PMID: 29185452

3. Woodley, KT. et al. (2019) EMBO reports 20, e47472. PMID: 31402609

4. Lu, Y. et al. (2019) Science (New York, N.Y.) 366, 460-467. PMID: 31649195

5. Dixon, CL. et al. (2021) Journal of lipid research 62, 100097. PMID: 34293401

6. Zheng, S. et al. (2023) Molecular cell 83, 4570-4585.e7. PMID: 38092000

7. Balasubramanian, A. et al. (2024) Science immunology 9, eadn1452. PMID: 38530158

8. Du, G. et al. (2024) Nature 630, 437-446. PMID: 38599239

9. Brigidi, GS. et al. (2015) Nature communications 6, 8200. PMID: 26334723

10. Hao, JW. et al. (2020) Nature communications 11, 4765. PMID: 32958780

Note

For research use only.

Images

References

Datasheet

Anti-ZDHHC5 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-ZDHHC5 Polyclonal Antibody (HA293014)

Overview

Images

References

Datasheet

SDS