Anti-ZEBOV NP/Nucleoprotein Antibody (KZ51) (VK518023)

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50ug
100ug
1mg

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Overview
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Datasheet

Overview

Catalog No.

VK518023

Description

Anti-ZEBOV NP/Nucleoprotein Antibody (KZ51) [KZ51] (VK518023) is a human monoclonal antibody detecting Nucleoprotein in ELISA, IF.

Species reactivity

Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)

Applications

ELISA, IF

Host species

Human

Isotype

IgG

Clone ID

KZ51

Clonality

Monoclonal

Target

NP, Nucleoprotein, Nucleocapsid protein

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P18272

RRID

Anti-ZEBOV NP/Nucleoprotein Antibody (KZ51) (abinScience Cat# VK518023, RRID:AB_3727247)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Nucleoprotein (NP) is a ~83 kDa protein. Oligomerizes into helical capsid to encapsidate the viral genome, protecting it from nucleases and the cellular innate immune response. VP35 binds to and stabilizes monomeric NP, keeping it soluble. Upon virus replication, NP is recruited to bind cooperatively viral genomic RNA and VP35 is released. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. The nucleocapsid is helical with a pitch of 10.81 NP per turn and a diameter of about 22nm.

1. Johnson, RF. et al. (2006) Virology journal 3, 31. PMID: 16719918

2. Leung, DW. et al. (2015) Cell reports 11, 376-89. PMID: 25865894

3. Kirchdoerfer, RN. et al. (2015) Cell reports 12, 140-149. PMID: 26119732

4. Sugita, Y. et al. (2018) Nature 563, 137-140. PMID: 30333622

5. Wan, W. et al. (2017) Nature 551, 394-397. PMID: 29144446

6. Beniac, DR. et al. (2012) PloS one 7, e29608. PMID: 22247782

7. Kirchdoerfer, RN. et al. (2016) PLoS pathogens 12, e1005937. PMID: 27755595

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-ZEBOV NP/Nucleoprotein Antibody (KZ51)

References

Datasheet

Anti-ZEBOV NP/Nucleoprotein Antibody (KZ51).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-ZEBOV NP/Nucleoprotein Antibody (KZ51) (VK518023)

Overview

Images

References

Datasheet

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