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Anti-ZEBOV VP24 Polyclonal Antibody (VK095014)

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Overview
Catalog No.VK095014
Description
Anti-ZEBOV VP24 Polyclonal Antibody (VK095014) is a rabbit polyclonal antibody detecting VP24 in ELISA, IHC, WB. Suitable for Zaire ebolavirus.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityZaire ebolavirus (ZEBOV) (Zaire Ebola virus)
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant ZEBOV VP24 (Met1-Ile251).
Target Membrane-associated protein VP24, Ebola VP24, eVP24, VP24
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q6V1Q3, Q05322, O11459
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Membrane-associated protein VP24 is a ~28 kDa protein. Prevents the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta) and IFN-gamma signaling pathways. Blocks the IFN-induced nuclear accumulation of host phosphorylated STAT1 by interacting with the STAT1-binding region of host importins. Alternatively also interacts directly with host STAT1 and may additionally inhibit its non-phosphorylated form. Plays a role in assembly of viral nucleocapsid and virion budding. May act as a minor matrix protein that plays a role in assembly of viral nucleocapsid and virion budding.

Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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