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Anti-Zebrafish akt1 Polyclonal Antibody (ZA455014)

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Overview
Catalog No.ZA455014
Description
Anti-Zebrafish akt1 Polyclonal Antibody (ZA455014) is a rabbit polyclonal antibody detecting akt1 in ELISA, IHC, WB. Suitable for Danio rerio, Mus musculus, Bovine, Xenopus laevis, and Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat, Zebrafish
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Zebrafish akt1 (Gly222-Lys395).
Target LOW QUALITY PROTEIN: RAC-alpha serine/threonine-protein kinase [Danio aesculapii], akt1, Protein Kinase B-like pleckstrin homology (PH) domain; cd01241, phosphoinositide binding site [chemical binding], The protein kinase superfamily is mainly composed of the catalytic domains of serine/threonine-specific and tyrosine-specific protein kinases. It also includes RIO kinases, which are atypical serine protein kinases, aminoglycoside phosphotransferases; cl21453, ATP binding site [chemical binding], The sequence of the model RefSeq protein was modified relative to its source genomic sequence to represent the inferred CDS: inserted 4 bases in 4 codons; substituted 1 base at 1 genomic stop codon
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession XP_056332579.1
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesLOW QUALITY PROTEIN: RAC-alpha serine/threonine-protein kinase, akt1
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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