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Anti-ZNF322 Polyclonal Antibody (HB474014)

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Overview
Catalog No.HB474014
Description
Anti-ZNF322 Polyclonal Antibody (HB474014) is a rabbit polyclonal antibody detecting Zinc finger protein 322; Zinc finger protein 322A; Zinc finger protein 388; Zinc finger protein 489; ZNF322; ZNF322A; ZNF388; ZNF489 in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human ZNF322 (Gly40-Glu376).
Target Zinc finger protein 322; Zinc finger protein 322A; Zinc finger protein 388; Zinc finger protein 489; ZNF322; ZNF322A; ZNF388; ZNF489
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q6U7Q0, Q8BZ89
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Zinc finger protein 322 (ZNF322) is a ~46 kDa protein. Transcriptional activator. Important for maintenance of pluripotency in embryonic stem cells. Binds directly to the POU5F1 distal enhancer and the NANOG proximal promoter, and enhances expression of both genes. Can also bind to numerous other gene promoters and regulates expression of many other pluripotency factors, either directly or indirectly. Promotes inhibition of MAPK signaling during embryonic stem cell differentiation.

1. Li, Y. et al. (2004) Biochemical and biophysical research communications 325, 1383-92. PMID: 15555580
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-ZNF322 Polyclonal Antibody

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