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CHO Human GPRC5D Stable Cell Line (HP458828)

CHO Human GPRC5D Stable Cell Line
CHO Human GPRC5D Stable Cell Line
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Overview
Catalog No.HP458828
Description
CHO Human GPRC5D Stable Cell Line (HP458828) is a stable cell line expressing GPRC5D. Engineered for consistent, high-level target expression in research applications.
Highlights
  • Stable Expression — Engineered for consistent, high-level target expression.
AccessionQ9NZD1
ApplicationsFCM
SpeciesHuman
Host species CHO
Target GPRC5D
Detection method Colorimetric
Sample type Plasma, Serum
Assay type Quantitative
Range 1.56 - 100 ng/mL
Sensitivity 0.25 ng/mL
Recovery 80-120%
Properties Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
Shipping 2-8 ℃
Stability and Storage Frozen in liquid nitrogen.
Specifications 1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
Background

G-protein coupled receptor family C group 5 member D (GPRC5D) is a ~38 kDa protein. G-protein coupled receptor involved in hard keratin expression and likely plays a role in the development of hair and nails.

Note For research use only. This stable cell line expresses GFP as a reporter marker. GFP is expressed independently of the target protein and is not fused to the target protein.
Images
  • CHO Human GPRC5D Stable Cell Line

    Flow cytometry

    Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human GPRC5D monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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