Evolocumab ELISA Kit (DV275018)

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Overview

Catalog No.	DV275018
Description	Evolocumab ELISA Kit (DV275018) — Quantitative sandwich ELISA for Evolocumab. Sample: Plasma, Serum. Range: 78.125 - 5, 000 ng/mL. Highlights ●Sandwich Format — High specificity, reliable quantification. ●Ready-to-Use — Complete kit with pre-coated plates included.
Sample type	Plasma, Serum
Range	78.125 - 5, 000 ng/mL
Accession	Q8NBP7
Applications	ELISA
Detection method	Colorimetric
Assay type	Quantitative
Recovery	80-120%
Shipping	2-8 ℃
Stability and Storage	The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 10% prior to the expiration date under appropriate storage condition.
Specifications	Evolocumab
Alternate Names	AMG 145, Anti-PCSK9, 1256937-27-5
Background	Proprotein convertase subtilisin/kexin type 9 (Evolocumab) is a ~74 kDa protein. Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments. Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation. 1. Poirier, S. et al. (2008) The Journal of biological chemistry 283, 2363-72. PMID: 18039658 2. Nassoury, N. et al. (2007) Traffic (Copenhagen, Denmark) 8, 718-32. PMID: 17461796 3. Fan, D. et al. (2008) Biochemistry 47, 1631-9. PMID: 18197702 4. Mayer, G. et al. (2008) The Journal of biological chemistry 283, 31791-801. PMID: 18799458 5. Chen, Y. et al. (2011) Biochemical and biophysical research communications 415, 515-8. PMID: 22074827 6. Jonas, MC. et al. (2008) EMBO reports 9, 916-22. PMID: 18660751
Note	For Research Use Only.