HEK293T Human CD184/CXCR4 Stable Cell Line (HX206818)

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Overview

Catalog No.	HX206818
Description	HEK293T Human CD184/CXCR4 Stable Cell Line (HX206818) is a stable cell line expressing CD184/CXCR4. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
Accession	P61073
Applications	FCM
Species	Human
Host species	HEK293T
Target	CD184/CXCR4
Detection method	Colorimetric
Sample type	Plasma, Serum
Assay type	Quantitative
Range	1.56 - 100 ng/mL
Sensitivity	0.25 ng/mL
Recovery	80-120%
Properties	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
Shipping	2-8 ℃
Stability and Storage	Frozen in liquid nitrogen.
Specifications	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
Background	C-X-C chemokine receptor type 4 (CD184/CXCR4) is a ~39 kDa protein. Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase. CXCR4 is coupled to G(i) G alpha proteins and mediates inhibition of adenylate cyclase. Involved in the AKT signaling cascade. Plays a role in regulation of cell migration, e.g. 1. Brelot, A. et al. (1999) Journal of virology 73, 2576-86. PMID: 10074102 2. Gupta, SK. et al. (1999) Journal of immunology (Baltimore, Md. : 1950) 163, 2368-72. PMID: 10452968 3. Cheng, ZJ. et al. (2000) The Journal of biological chemistry 275, 2479-85. PMID: 10644702 4. Brelot, A. et al. (2000) The Journal of biological chemistry 275, 23736-44. PMID: 10825158 5. Veldkamp, CT. et al. (2008) Science signaling 1, ra4. PMID: 18799424 6. Busillo, JM. et al. (2010) The Journal of biological chemistry 285, 7805-17. PMID: 20048153 7. Malik, R. et al. (2010) Molecular biology of the cell 21, 2529-41. PMID: 20505072 8. Cao, Y. et al. (2015) Leukemia 29, 169-76. PMID: 24912431 9. Lear, T. et al. (2017) American journal of physiology. Cell physiology 313, C584-C592. PMID: 28978524 10. Bleul, CC. et al. (1996) Nature 382, 829-33. PMID: 8752280
Note	For research use only. This stable cell line expresses GFP as a reporter marker. GFP is expressed independently of the target protein and is not fused to the target protein.

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Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CXCR4 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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