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HEK293T Human CD185/CXCR5 Stable Cell Line (HB160818)

HEK293T Human CD185/CXCR5 Stable Cell Line
HEK293T Human CD185/CXCR5 Stable Cell Line
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  • Datasheet

Overview
Catalog No.HB160818
Description
HEK293T Human CD185/CXCR5 Stable Cell Line (HB160818) is a stable cell line expressing CD185/CXCR5. Engineered for consistent, high-level target expression in research applications.
Highlights
  • Stable Expression — Engineered for consistent, high-level target expression.
AccessionP32302
ApplicationsFCM
SpeciesHuman
Host species HEK293T
Target CD185/CXCR5
Detection method Colorimetric
Sample type Plasma, Serum
Assay type Quantitative
Range 1.56 - 100 ng/mL
Sensitivity 0.25 ng/mL
Recovery 80-120%
Properties Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
Shipping 2-8 ℃
Stability and Storage Frozen in liquid nitrogen.
Specifications 1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
Background

C-X-C chemokine receptor type 5 (CD185/CXCR5) is a ~41 kDa protein. Cytokine receptor that binds to B-lymphocyte chemoattractant (BLC). Involved in B-cell migration into B-cell follicles of spleen and Peyer patches but not into those of mesenteric or peripheral lymph nodes. May have a regulatory function in Burkitt lymphoma (BL) lymphomagenesis and/or B-cell differentiation.

Note For research use only. This stable cell line expresses GFP as a reporter marker. GFP is expressed independently of the target protein and is not fused to the target protein.
Images
  • HEK293T Human CD185/CXCR5 Stable Cell Line

    Flow cytometry

    Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CXCR5 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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