HEK293T Human CD196/CCR6 Stable Cell Line (HW383818)

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Datasheet

Overview

Catalog No.	HW383818
Description	HEK293T Human CD196/CCR6 Stable Cell Line (HW383818) is a stable cell line expressing CD196/CCR6. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
Accession	P51684
Applications	FCM
Species	Human
Host species	HEK293T
Target	CD196/CCR6
Properties	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
Stability and Storage	Frozen in liquid nitrogen.
Specifications	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
Background	C-C chemokine receptor type 6 (CD196/CCR6) is a ~42 kDa protein. Receptor for the C-C type chemokine CCL20. Binds to CCL20 and subsequently transduces a signal by increasing the intracellular calcium ion levels. Although CCL20 is its major ligand it can also act as a receptor for non-chemokine ligands such as beta-defensins. Binds to defensin DEFB1 leading to increase in intracellular calcium ions and cAMP levels. Its binding to DEFB1 is essential for the function of DEFB1 in regulating sperm motility and bactericidal activity. 1. Baba, M. et al. (1997) The Journal of biological chemistry 272, 14893-8. PMID: 9169459 2. Röhrl, J. et al. (2010) The Journal of biological chemistry 285, 7028-34. PMID: 20068036 3. Lee, AY. et al. (2015) Cytokine 72, 97-101. PMID: 25585877 4. Diao, R. et al. (2014) Science translational medicine 6, 249ra108. PMID: 25122636 5. Ito, T. et al. (2011) Experimental cell research 317, 613-9. PMID: 21376174 6. Caballero-Campo, P. et al. (2014) Journal of cellular physiology 229, 68-78. PMID: 23765988

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Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD196 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

HEK293T Human CD196/CCR6 Stable Cell Line.pdf

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Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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C₁ × V₁ = C₂ × V₂

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C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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HEK293T Human CD196/CCR6 Stable Cell Line (HW383818)

Overview

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References

Datasheet

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