HEK293T Human CD198/CCR8 Stable Cell Line (HW444818)

Overview

Catalog No.	HW444818
Description	HEK293T Human CD198/CCR8 Stable Cell Line (HW444818) is a stable cell line expressing CD198/CCR8. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
Accession	P51685
Applications	FCM
Species	Human
Host species	HEK293T
Target	CD198/CCR8
Properties	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
Stability and Storage	Frozen in liquid nitrogen.
Specifications	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
Background	C-C chemokine receptor type 8 (CD198/CCR8) is a ~40 kDa protein. G protein-coupled receptor that can bind a variety of chemokines, such as CCL1, CCL8, CCL16, CCL18. Regulates monocyte and eosinophil chemotaxis. Undergoes internalization upon CCL18 binding, leading to induced migration and calcium flux of highly polarized Th2 cells. In microglial cells, promotes phagocytosis with CCL18 through NF-kappa-B and Src signaling pathways. Stimulation of the CCL1-CCR8 signaling axis protects the gut from acute intestinal damage. 1. Islam, SA. et al. (2013) The Journal of experimental medicine 210, 1889-98. PMID: 23999500 2. Ding, D. et al. (2022) Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 42, 19-28. PMID: 35041514

Images

Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CCR8 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet