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HEK293T Human CD227/MUC1 Stable Cell Line (HB102818)

HEK293T Human CD227/MUC1 Stable Cell Line
HEK293T Human CD227/MUC1 Stable Cell Line
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  • Datasheet

Overview
Catalog No.HB102818
Description
HEK293T Human CD227/MUC1 Stable Cell Line (HB102818) is a stable cell line expressing CD227/MUC1. Engineered for consistent, high-level target expression in research applications.
Highlights
  • Stable Expression — Engineered for consistent, high-level target expression.
AccessionP15941
ApplicationsFCM
SpeciesHuman
Host species HEK293T
Target CD227/MUC1
Properties Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
Stability and Storage Frozen in liquid nitrogen.
Specifications 1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
Background

Mucin-1 (CD227/MUC1) is a ~122 kDa protein. The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.

Images
  • HEK293T Human CD227/MUC1 Stable Cell Line

    Flow cytometry

    Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human MUC1 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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