HEK293T Human CD272/BTLA Stable Cell Line (HV375818)

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Overview

Catalog No.	HV375818
Description	HEK293T Human CD272/BTLA Stable Cell Line (HV375818) is a stable cell line expressing CD272/BTLA. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
Accession	Q7Z6A9
Applications	FCM
Species	Human
Host species	HEK293T
Target	CD272/BTLA
Properties	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
Stability and Storage	Frozen in liquid nitrogen.
Specifications	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
Background	B- and T-lymphocyte attenuator (CD272/BTLA) is a ~32 kDa protein. Inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2. May interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14. In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state. In trans interactions, can predominate during adaptive immune response to provide survival signals to effector T cells. 1. Watanabe, N. et al. (2003) Nature immunology 4, 670-9. PMID: 12796776 2. Gavrieli, M. et al. (2003) Biochemical and biophysical research communications 312, 1236-43. PMID: 14652006 3. Sedy, JR. et al. (2005) Nature immunology 6, 90-8. PMID: 15568026 4. Cai, G. et al. (2008) Nature immunology 9, 176-85. PMID: 18193050 5. Cheung, TC. et al. (2009) Journal of immunology (Baltimore, Md. : 1950) 183, 7286-96. PMID: 19915044

Images

Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human BTLA monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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