HEK293T Human CD278/ICOS Stable Cell Line (HV702818)

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Overview

Catalog No.	HV702818
Description	HEK293T Human CD278/ICOS Stable Cell Line (HV702818) is a stable cell line expressing CD278/ICOS. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
Accession	Q9Y6W8
Applications	FCM
Species	Human
Host species	HEK293T
Target	CD278/ICOS
Properties	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
Stability and Storage	Frozen in liquid nitrogen.
Specifications	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
Background	Inducible T-cell costimulator (CD278/ICOS) is a ~22 kDa protein. Stimulatory receptor expressed in activated or antigen-experienced T-cells that plays an important role in the immune response. Upon binding to its ligand ICOSL expressed on antigen presenting cells (APCs), delivers costimulatory signals that enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines including IL10, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Also acts as a costimulatory receptor critical for the differentiation of T follicular regulatory cells upon immune challenges such as viral infection. Mechanistically, potentiates TCR-induced calcium flux by augmenting PLCG1 activation and actin remodeling. In addition, activates PI3K signaling pathways independently of calcium flux. 1. Hutloff, A. et al. (1999) Nature 397, 263-6. PMID: 9930702 2. Rujas, E. et al. (2020) Nature communications 11, 5066. PMID: 33033255 3. Pedros, C. et al. (2016) Nature immunology 17, 825-33. PMID: 27135603 4. Wan, Z. et al. (2020) Cellular & molecular immunology 17, 143-152. PMID: 30523347

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Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human ICOS monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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