HEK293T Human CD44 Stable Cell Line (HB183818)

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Overview

Catalog No.	HB183818
Description	HEK293T Human CD44 Stable Cell Line (HB183818) is a stable cell line expressing CD44. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
Accession	P16070
Applications	FCM
Species	Human
Host species	HEK293T
Target	CD44
Properties	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
Stability and Storage	Frozen in liquid nitrogen.
Specifications	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
Background	CD44 antigen is a ~81 kDa protein. Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment. Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection. Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases. Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion. Upon interaction with LGALS9 ligand, activates downstream signaling components including LCK, ERK and MAPK to promotes NK cell activation. 1. Vikesaa, J. et al. (2006) The EMBO journal 25, 1456-68. PMID: 16541107 2. Crosby, HA. et al. (2009) Journal of hepatology 51, 734-49. PMID: 19703720 3. Yoshida, T. et al. (2012) Pathology international 62, 463-70. PMID: 22726066 4. Funaro, A. et al. (1994) Human immunology 40, 267-78. PMID: 7528188 5. Casalino-Matsuda, SM. et al. (2009) American journal of respiratory cell and molecular biology 40, 277-85. PMID: 18757307 6. Midgley, AC. et al. (2013) The Journal of biological chemistry 288, 14824-38. PMID: 23589287 7. Bourguignon, LY. et al. (2004) The Journal of biological chemistry 279, 29654-69. PMID: 15123640 8. Rahmati, A. et al. (2023) Frontiers in immunology 14, 1131379. PMID: 37006235

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Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD44 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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