HEK293T Human ERBB3/HER3 Stable Cell Line (HB116818)

Overview

Catalog No.	HB116818
Description	HEK293T Human ERBB3/HER3 Stable Cell Line (HB116818) is a stable cell line expressing ERBB3/HER3. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
Accession	P21860
Applications	FCM
Species	Human
Host species	HEK293T
Target	ERBB3/HER3
Properties	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
Stability and Storage	Frozen in liquid nitrogen.
Specifications	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
Background	Receptor tyrosine-protein kinase erbB-3 (ERBB3/HER3) is a ~148 kDa protein. Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins. Binds to neuregulin-1 (NRG1) and is activated by it; ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase. May also be activated by CSPG5. Involved in the regulation of myeloid cell differentiation. 1. Ieguchi, K. et al. (2010) The Journal of biological chemistry 285, 31388-98. PMID: 20682778 2. Kinugasa, Y. et al. (2004) Biochemical and biophysical research communications 321, 1045-9. PMID: 15358134 3. Braunstein, EM. et al. (2016) Leukemia 30, 2242-2245. PMID: 27416908

Images

Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human HER3 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet