HEK293T Human FOLR1 Stable Cell Line (HB825818)

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Overview

Catalog No.	HB825818
Description	HEK293T Human FOLR1 Stable Cell Line (HB825818) is a stable cell line expressing FOLR1. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
Accession	P15328
Applications	FCM
Species	Human
Host species	HEK293T
Target	FOLR1
Properties	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
Stability and Storage	Frozen in liquid nitrogen.
Specifications	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
Background	Folate receptor alpha (FOLR1) is a ~29 kDa protein. Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH. Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release. Required for normal embryonic development and normal cell proliferation. FOLR1 is the therapeutic target of mirvetuximab soravtansine (Elahere). 1. Zhao, R. et al. (2009) The Journal of biological chemistry 284, 4267-74. PMID: 19074442 2. Chen, C. et al. (2013) Nature 500, 486-9. PMID: 23851396 3. Wibowo, AS. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 15180-8. PMID: 23934049 4. Lacey, SW. et al. (1989) The Journal of clinical investigation 84, 715-20. PMID: 2527252 5. Orr, RB. et al. (1994) Cancer research 54, 3905-11. PMID: 8033114 6. Rijnboutt, S. et al. (1996) The Journal of cell biology 132, 35-47. PMID: 8567728

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Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human FOLR1 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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