HEK293T Human GPC3/Glypican 3 Stable Cell Line (HW310818)

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Overview

Catalog No.	HW310818
Description	HEK293T Human GPC3/Glypican 3 Stable Cell Line (HW310818) is a stable cell line expressing GPC3/Glypican 3. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
Accession	P51654
Applications	FCM
Species	Human
Host species	HEK293T
Target	GPC3/Glypican 3
Properties	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
Stability and Storage	Frozen in liquid nitrogen.
Specifications	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
Background	Glypican-3 (GPC3/Glypican 3) is a ~65 kDa protein. Cell surface proteoglycan. Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins. Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation. Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands. Positively regulates the non-canonical Wnt signaling pathway. 1. De Cat, B. et al. (2003) The Journal of cell biology 163, 625-35. PMID: 14610063 2. Capurro, MI. et al. (2005) The Journal of biological chemistry 280, 41201-6. PMID: 16227623 3. Capurro, M. et al. (2014) Journal of cell science 127, 1565-75. PMID: 24496449 4. Davoodi, J. et al. (2007) Proteomics 7, 2300-10. PMID: 17549790

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Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human GPC3 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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