HEK293T Human CD46 Stable Cell Line (HB735818)

Overview

Catalog No.	HB735818
Description	HEK293T Mouse CD46 Stable Cell Line (HB735818) is a stable cell line expressing CD46. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
Accession	P15529
Applications	FCM
Species	Mouse
Host species	HEK293T
Target	CD46
Detection method	Colorimetric
Sample type	Plasma, Serum
Assay type	Quantitative
Range	1.56 - 100 ng/mL
Sensitivity	0.25 ng/mL
Recovery	80-120%
Properties	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
Shipping	2-8 ℃
Stability and Storage	Frozen in liquid nitrogen.
Specifications	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
Background	Membrane cofactor protein (CD46) is a ~43 kDa protein. Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity.
Note	For research use only

Images

Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD46 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet