HEK293T SARS-CoV-2 Spike (wt) Stable Cell Line (VK474828)

Overview

Catalog No.	VK474828
Description	HEK293T SARS-CoV-2 Spike (wt) Stable Cell Line (VK474828) is a stable cell line expressing SARS-CoV-2 Spike (wt). Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
Accession	P0DTC2 [Ser13-Pro1213]
Applications	FCM
Species	Viral (SARS-CoV-2)
Host species	HEK293T
Target	SARS-CoV-2 Spike (wt)
Properties	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
Stability and Storage	Frozen in liquid nitrogen.
Specifications	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium

Images

Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-SARS-CoV-2 Spike glycoprotein monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Overview