InVivoMAb Anti-Human Apolipoprotein E/ApoE Antibody (HJ15.4) (HF739040)

Name: InVivoMAb Anti-Human Apolipoprotein E/ApoE Antibody (HJ15.4)
Brand: abinScience
SKU: HF739040
Price: 370 USD
Availability: InStock

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100ug
1mg

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.	HF739040
Description	InVivoMAb Anti-Human APOE2/3/4 Antibody (HJ15.4) [HJ15.4] (HF739040) is a mouse monoclonal antibody detecting Apo-E in ELISA, FuncS. Suitable for Human.
Species reactivity	Human
Applications	ELISA, FuncS
Host species	Mouse
Isotype	IgG2a, kappa
Clone ID	HJ15.4
Clonality	Monoclonal
Target	Apo-E, Apolipoprotein E, APOE
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Associated products	pH 7.4 Dilution Buffer
Purification	Protein A/G purified from cell culture supernatant.
Accession	P02649
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background	Apolipoprotein E (APOE2/3) is a ~36 kDa protein. APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance. Apolipoproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma. As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL). It also binds a wide range of cellular receptors including the LDL receptor/LDLR, the LDL receptor-related proteins LRP1, LRP2 and LRP8 and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles. 1. Krimbou, L. et al. (2004) Journal of lipid research 45, 839-48. PMID: 14754908 2. Arnon, R. et al. (1991) Biochimica et biophysica acta 1085, 336-42. PMID: 1911868 3. Marcel, YL. et al. (1983) Biochimica et biophysica acta 750, 411-7. PMID: 6860692 4. Sehayek, E. et al. (1991) The Journal of biological chemistry 266, 18259-67. PMID: 1917954 5. Verghese, PB. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, E1807-16. PMID: 23620513 6. Kowal, RC. et al. (1989) Proceedings of the National Academy of Sciences of the United States of America 86, 5810-4. PMID: 2762297 7. Ji, ZS. et al. (1997) The Journal of biological chemistry 272, 31285-92. PMID: 9395455 8. Li, X. et al. (2003) Biochemistry 42, 10406-17. PMID: 12950167
Note	For research use only. Not suitable for clinical or therapeutic use.

Images

Western blot

Western blot analysis was performed using anti-APOE4 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human APOE4 (Catalog No: HF739022)
Lane 2: negative control
Flow cytometry

Flow-cytometry using anti-human APOE antibody. HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human APOE monoclonal antibody (Catalog HF739040, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for InVivoMAb Anti-Human APOE2/3/4 Antibody (HJ15.4)