InVivoMAb Anti-Human CD40/TNFRSF5 Antibody (Iv0136) (HB782010)

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Overview

Catalog No.	HB782010
Description	InVivoMAb Anti-Human CD40/TNFRSF5 Antibody (Iv0136) [Iv0136] (HB782010) is a human monoclonal antibody detecting Tumor necrosis factor receptor superfamily member 5 in ELISA, Neutralization. Suitable for Human.
Species reactivity	Human
Applications	ELISA, Neutralization
Host species	Human
Isotype	IgG1, kappa
Clone ID	Iv0136
Clonality	Monoclonal
Target	Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, TNFRSF5, CDw40, CD40
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P25942
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background	Tumor necrosis factor receptor superfamily member 5 (CD40/TNFRSF5) is a ~30 kDa protein. Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion. 1. Takada, YK. et al. (2019) Journal of immunology (Baltimore, Md. : 1950) 203, 1383-1391. PMID: 31331973
Note	For research use only. Not suitable for clinical or therapeutic use.

Images

Bioactivity

Detects CD40/TNFRSF5 in indirect ELISAs.
SDS-PAGE

SDS-PAGE for InVivoMAb Anti-Human CD40/TNFRSF5 Antibody (Iv0136).
Flow CytoMetry

Flow-cytometry using APC anti-human CD40 antibody. U2OS cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD40 monoclonal antibody (Catalog: HB782010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using FITC anti-human CD40 antibody. U2OS cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD40 monoclonal antibody (Catalog: HB782010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.