InVivoMAb anti-Mouse TIM-3 Antibody (B8.2C12) (MV029010)

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Spec:

100ug
1mg

Number:

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Datasheet
SDS

Overview

Catalog No.

MV029010

Description

InVivoMab Anti-Mouse CD366/HAVCR2/TIM-3 Antibody (B8.2C12) [B8.2C12] (MV029010) is a rat monoclonal antibody detecting CD366 in ELISA, Bioactivity: FACS, Functional assay, Research in vivo. Suitable for Mouse.

Highlights

●Multi-Application — Validated across multiple applications.

Species reactivity

Mouse

Applications

ELISA, Bioactivity: FACS, Functional assay, Research in vivo

Host species

Rat

Isotype

IgG1, kappa

Clone ID

B8.2C12

Clonality

Monoclonal

Target

CD366, HAVcr-2, Havcr2, Hepatitis A virus cellular receptor 2 homolog, T-cell immunoglobulin and mucin domain-containing protein 3, T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, TIM-3, TIMD-3, Tim3, Timd3

Endotoxin level

< 10 EU/mg

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q8VIM0

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Hepatitis A virus cellular receptor 2 homolog (CD366/HAVCR2) is a ~30 kDa protein. Cell surface receptor implicated in modulating innate and adaptive immune responses. Generally accepted to have an inhibiting function. Reports on stimulating functions suggest that the activity may be influenced by the cellular context and/or the respective ligand. Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance.

1. Anderson, AC. et al. (2007) Science (New York, N.Y.) 318, 1141-3. PMID: 18006747

2. Monney, L. et al. (2002) Nature 415, 536-41. PMID: 11823861

3. Sabatos, CA. et al. (2003) Nature immunology 4, 1102-10. PMID: 14556006

4. Lee, J. et al. (2011) Molecular and cellular biology 31, 3963-74. PMID: 21807895

5. Rangachari, M. et al. (2012) Nature medicine 18, 1394-400. PMID: 22863785

6. Jayaraman, P. et al. (2010) The Journal of experimental medicine 207, 2343-54. PMID: 20937702

7. Gorman, JV. et al. (2014) Journal of immunology (Baltimore, Md. : 1950) 192, 3133-42. PMID: 24567532

8. DeKruyff, RH. et al. (2010) Journal of immunology (Baltimore, Md. : 1950) 184, 1918-30. PMID: 20083673

9. Nakayama, M. et al. (2009) Blood 113, 3821-30. PMID: 19224762

Note

For research use only.

Images

References

Datasheet

InVivoMAb anti-Mouse TIM-3 Antibody (B8.2C12) .pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

InVivoMAb anti-Mouse TIM-3 Antibody (B8.2C12) (MV029010)

Overview

Images

References

Datasheet

SDS

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