Mouse Anti-Human BMP2 Recombinant Antibody (Iv0058) (HY576023)

Name: Mouse Anti-Human BMP2 Recombinant Antibody (Iv0058)
Brand: abinScience
SKU: HY576023
Price: 110 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HY576023

Species reactivity

Human

Applications

ELISA, FCM, Neutralization

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

Iv0058

Clonality

Monoclonal

Target

BMP-2, BMP2A, Bone morphogenetic protein 2A, Bone morphogenetic protein 2, BMP2, BMP-2A

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P12643

RRID

Mouse Anti-Human BMP2 Antibody (Iv0058) (abinScience Cat# HY576023, RRID:AB_3729371)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Bone morphogenetic protein 2 (BMP2) is a ~44 kDa protein. Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including cardiogenesis, neurogenesis, and osteogenesis. Induces cartilage and bone formation. Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2. Once all three components are bound together in a complex at the cell surface, BMPR2 phosphorylates and activates BMPR1A. In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes.

1. Lavery, K. et al. (2008) The Journal of biological chemistry 283, 20948-58. PMID: 18436533

2. Tanaka, K. et al. (2014) Calcified tissue international 94, 454-64. PMID: 24362451

3. Seeherman, HJ. et al. (2019) Science translational medicine 11. PMID: 31019025

4. Wozney, JM. et al. (1988) Science (New York, N.Y.) 242, 1528-34. PMID: 3201241

5. Keller, S. et al. (2004) Nature structural & molecular biology 11, 481-8. PMID: 15064755

6. Weber, D. et al. (2007) BMC structural biology 7, 6. PMID: 17295905

7. Liu, F. et al. (1995) Molecular and cellular biology 15, 3479-86. PMID: 7791754

8. Wang, CY. et al. (2020) Blood 135, 453-456. PMID: 31800957

9. Tsukahara, S. et al. (2006) The Biochemical journal 398, 595-603. PMID: 16771708

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Mouse Anti-Human BMP2 Antibody (Iv0058).
Flow CytoMetry

Flow-cytometry using PE anti-human BMP4 antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human BMP4 monoclonal antibody (Catalog: HY576023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Mouse Anti-Human BMP2 Recombinant Antibody (Iv0058).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Mouse Anti-Human BMP2 Recombinant Antibody (Iv0058) (HY576023)

Overview

Images

References

Datasheet

SDS

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