ASFV NP419L Recombinant Protein (N-His) (VK110022)

Datasheet Share product Copy info

Price(USD): $

Spec:

50ug
100ug
1mg

Number:

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.	VK110022
Description	Recombinant ASFV NP419L Protein, N-His (VK110022) expressed in E. coli, spanning Met1-Ile419. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P35970
Protein length	Met1-Ile419
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	African swine fever virus
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	DNA ligase, EC:6.5.1.1, Polydeoxyribonucleotide synthase [ATP], LIG
Background	DNA ligase is a ~48 kDa protein. Very low-fidelity DNA ligase that seals nicks in double-stranded DNA during DNA repair. Together with the viral repair DNA polymerase X, fills the single nucleotide gaps generated by the AP endonuclease. It is not essential for viral replication and recombination. Displays a very low adenylation activity towards DNA with 3'-dideoxy- or 3'-amino-terminated nicks compared to regular nick DNA. 1. Lamarche, BJ. et al. (2005) Biochemistry 44, 8408-17. PMID: 15938630
Note	For research use only

Images

SDS-PAGE

SDS-PAGE for Recombinant ASFV NP419L Protein, N-His

References

Datasheet

ASFV NP419L Recombinant Protein (N-His).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

ASFV NP419L Recombinant Protein (N-His) (VK110022)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us