

| Catalog No. | JN979012 |
|---|---|
| Description |
Recombinant Aspergillus niger gox Protein, N-His (JN979012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
|
| Expression system | E. coli |
| Accession | P13006 |
| Protein length | Ser23-Gln605 |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Species | Aspergillus niger |
| Nature | Recombinant |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >90% as determined by SDS-PAGE. |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternate Names | Beta-D-glucose:oxygen 1-oxido-reductase, EC:1.1.3.4, GOD, GOx, Glucose oxidase, gox |
| Background | Glucose oxidase is a ~65 kDa protein. Glucose oxidase catalyzes the oxidation of beta-D-glucose to D-glucono-delta-lactone and hydrogen peroxide in the presence of molecular oxygen. D-glucono-delta-lactone is sequentially hydrolyzed by lactonase to D-gluconic acid, and the resulting hydrogen peroxide is hydrolyzed by catalase to oxygen and water (PubMed:14188176, PubMed:14257628, PubMed:14299649, PubMed:15450808, PubMed:2076553, PubMed:2406261, PubMed:24283586, PubMed:28631058, PubMed:28939970, PubMed:34500289, PubMed:7826581, Ref.4, Ref.5). The activity shows high specificity to beta-D-glucose, with very low to no activity towards L-glucose, 2-deoxy-D-glucose, 3-deoxy-D-glucose, 4-deoxy-D-glucose, 5-deoxy-D-glucose, 6-deoxy-D-glucose, 3-O-methyl-D-glucose, 4-O-methyl-D-glucose, 6-O-methyl-D-glucose, 4,6-O-benzylidene-D-glucose, 5-thio-5-deoxy-D-glucose, D-mannose, D-allose, D-galactose, D-fructose, D-arabinose, D-xylose, trehalose, melibiose, L-mannomethylose, lactose, sucrose or 1,5-anhydro-D-glucitol (PubMed:14188176, PubMed:14257628, PubMed:24283586, Ref.5). 1. PAZUR, JH. et al. (1964) Biochemistry 3, 578-83. PMID: 14188176 2. GIBSON, QH. et al. (1964) The Journal of biological chemistry 239, 3927-34. PMID: 14257628 3. SWOBODA, BE. et al. (1965) The Journal of biological chemistry 240, 2209-15. PMID: 14299649 4. Karmali, K. et al. (2004) Analytical biochemistry 333, 320-7. PMID: 15450808 5. Whittington, H. et al. (1990) Current genetics 18, 531-6. PMID: 2076553 6. Frederick, KR. et al. (1990) The Journal of biological chemistry 265, 3793-802. PMID: 2406261 7. Meng, Y. et al. (2014) Letters in applied microbiology 58, 393-400. PMID: 24283586 8. Khadivi Derakshan, F. et al. (2017) Molecular biotechnology 59, 307-314. PMID: 28631058 9. Zhu, X. et al. (2018) Biotechnology letters 40, 103-110. PMID: 28939970 10. Zhong, P. et al. (2022) Food chemistry 370, 130901. PMID: 34500289 |
| Note | For research use only |
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