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Bovine EGF Recombinant Protein (N-Fc) (BF557011)

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Overview
Catalog No.BF557011
Description
Recombinant Bovine EGF Protein, N-Fc (BF557011) expressed in Mammalian Cells, spanning Lys979-His1031. Purity: >90% by SDS-PAGE.
Highlights
  • ●Fc Fusion — Enhanced stability with Protein A/G purification compatibility.
  • ●Mammalian Expression — Proper folding and native glycosylation.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Expression systemMammalian cells
AccessionXP_059743690.1
Protein lengthLys979-His1031
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesBos taurus (Bovine)
Nature Recombinant
Endotoxin level <0.1 EU/ug as determined by LAL method.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 33.93 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesEGF, Epidermal growth factor, Pro-epidermal growth factor, Urogastrone
Background

Pro-epidermal growth factor (EGF) is a ~133 kDa protein. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro.

1. Hermann, PM. et al. (2000) The Journal of neuroscience : the official journal of the Society for Neuroscience 20, 6355-64. PMID: 10964941
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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