Feline NT-ProBNP/NPPB Recombinant Protein (N-His-SUMO) (FB840022)

Name: Feline NT-ProBNP/NPPB Recombinant Protein (N-His-SUMO)
Brand: abinScience
SKU: FB840022
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	FB840022
Description	Recombinant Cat NT-ProBNP/NPPB Protein, N-His-SUMO (FB840022) expressed in E. coli, spanning His27-Arg98. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9GLK4
Protein length	His27-Arg98
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Felis catus (Cat) (Felis silvestris catus)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	20.04 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Natriuretic peptides B, Brain natriuretic factor prohormone, preproBNP, proBNP, Gamma-brain natriuretic peptide, Iso-ANP, Brain natriuretic peptide 35, BNP-35, Brain natriuretic peptide 29, BNP-29, NPPB, BNP
Background	Natriuretic peptides B (NT-ProBNP) is a ~14 kDa protein. Cardiac hormone that plays a key role in mediating cardio-renal homeostasis. May also function as a paracrine antifibrotic factor in the heart. Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses. Involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Binds the clearance receptor NPR3.
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Cat NT-ProBNP/NPPB Protein, N-His-SUMO

References

FB840022-SDS-PAGE-1.jpg

Datasheet

Feline NT-ProBNP/NPPB Recombinant Protein (N-His-SUMO).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Feline NT-ProBNP/NPPB Recombinant Protein (N-His-SUMO) (FB840022)

Overview

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References

Datasheet

SDS

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