Clostridium perfringens plc/Phospholipase C/Alpha-toxin Recombinant Protein (C-His) (JN878012)

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Catalog No.	JN878012
Description	Recombinant Clostridium perfringens plc/Phospholipase C/Alpha-toxin Protein, C-His (JN878012) expressed in E. coli, spanning Ala28-Lys398. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P0C216
Protein length	Ala28-Lys398
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Clostridium perfringens
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Phospholipase C, PLC, Alpha-toxin, Hemolysin, Lecithinase, Phosphatidylcholine cholinephosphohydrolase, cpa
Background	Phospholipase C (plc/Alpha-toxin) is a ~45 kDa protein. Bacterial hemolysins are exotoxins that attack blood cell membranes and cause cell rupture. Constitutes an essential virulence factor in gas gangrene. Binds to eukaryotic membranes where it hydrolyzes both phosphatidylcholine and sphingomyelin. The diacylglycerol produced can activate both the arachidonic acid pathway, leading to modulation of the inflammatory response cascade and thrombosis, and protein kinase C, leading to activation of eukaryotic phospholipases and further membrane damage. Acts on human and mouse erythrocytes, but not on rabbit or horse erythrocytes.
Note	For research use only.

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SDS-PAGE for Recombinant Clostridium perfringens plc/Phospholipase C/Alpha-toxin Protein, C-His

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JN878012-SDS-PAGE-1.jpg

Datasheet

Clostridium perfringens plc/Phospholipase C/Alpha-toxin Recombinant Protein (C-His).pdf

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Clostridium perfringens plc/Phospholipase C/Alpha-toxin Recombinant Protein (C-His) (JN878012)

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References

Datasheet

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