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Canine IL-2 R beta Recombinant Protein (N-His) (CB912012)

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Overview
Catalog No.CB912012
Description
Recombinant Dog CD122/IL2RB Protein, N-His (CB912012) expressed in E. coli, spanning Ser26-Ser241. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Expression systemE. coli
AccessionNP_001273779.1
Protein lengthSer26-Ser241
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesCanis lupus familiaris (Dog) (Canis familiaris)
Nature Recombinant
Endotoxin level Remove endotoxin and sterilization is required for cell assay.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 27.14 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesCD122, High affinity IL-2 receptor subunit beta, IL-2 receptor subunit beta, IL-2R subunit beta, IL-2RB, IL15RB, IL2RB, Interleukin-15 receptor subunit beta, Interleukin-2 receptor subunit beta, p70-75, p75
Background

Interleukin-2 receptor subunit beta (CD122/IL2RB) is a ~61 kDa protein. Receptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15.

1. Ratthé, C. et al. (2004) Journal of leukocyte biology 76, 162-8. PMID: 15123770
2. Zhang, Z. et al. (2019) The Journal of experimental medicine 216, 1311-1327. PMID: 31040185
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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