Escherichia coli tetR Recombinant Protein (N-His) (JN866011)

Name: Escherichia coli tetR Recombinant Protein (N-His)
Brand: abinScience
SKU: JN866011
Price: 298 USD
Availability: InStock

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Overview

Catalog No.	JN866011
Description	_x005F_x000D_ _x005F_x000D_ _x005F_x000D_ _x005F_x000D_ _x005F_x000D_ _x005F_x000D_ Recombinant Escherichia coli tetR Protein, N-His (JN866011) expressed in Mammalian Cells, spanning Met1-Ser207. Purity: >90% by SDS-PAGE. _x005F_x000D_ _x005F_x000D_ _x005F_x000D_ Highlights _x005F_x000D_ _x005F_x000D_ _x005F_x000D_ ●His-Tagged — N-terminal 6×His tag for IMAC purification. _x005F_x000D_ ●Mammalian Expression — Proper folding and native glycosylation. _x005F_x000D_ ●High Purity — >90% purity verified by SDS-PAGE. _x005F_x000D_ _x005F_x000D_ _x005F_x000D_
Expression system	Mammalian cells
Accession	P04483
Protein length	Met1-Ser207
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Escherichia coli
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	25.19 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Tetracycline repressor protein class B from transposon Tn10, tetR
Background	Tetracycline repressor protein class B from transposon Tn10 is a ~23 kDa protein. TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites. _x005F_x000D_
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Escherichia coli tetR Protein, N-His

References

JN866011-SDS-PAGE-1.jpg

Datasheet

Escherichia coli tetR Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Escherichia coli tetR Recombinant Protein (N-His) (JN866011)

Overview

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References

Datasheet

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